河北省猪腹泻相关病毒的检测及猪流行性腹泻病毒S基因遗传变异分析

doi:10.11843/j.issn.0366-6964.2018.03.017

Abstract

Abstract:

To understand the epidemiology of the pigs diarrhea virus in Hebei province, a total of 1 855 clinical samples were collected from April, 2016 to February, 2017 and subjected to detection of porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PoRV) by real-time PCR. Spike (S) genes of nine amplified PEDV were sequenced and analyzed. The results showed that positive rate of diarrhea was 9.70% and highest in winter and spring seasons. The positive rates of PEDV of total diarrhea samples, piglets diarrhea samples and fattening pigs diarrhea samples were 13.89%, 19.28% and 17.24%, respectively. The phylogenetic analysis of PEDV S genes amino acid sequence showed that the detected 9 S genes from Hebei were distributed in two branches of group Ⅱ. The detail analysis showed several unique amino acid mutations including deletions and insertions in the 9 S genes. These results indicate PEDV infection has fell on piglets but rise on fattening pigs, and the genetic diversity and mutations of S genes were potentially cause alternations of pathogenesis and antigenicity of pandemic PEDV in Hebei province.

CLC Number:

S852.65

ZHANG Ruo-xi, ZHANG Zhi, GU Wen-yuan, LIU Tian-ju, LI Chong, WANG Jian-chang, LI Bin, YUAN Wan-zhe, WANG Yu-qing, HAN Qing-an. Detection of Porcine Diarrhea Associated Virus and Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus in Hebei Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 597-605.

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https://www.xmsyxb.com/EN/Y2018/V49/I3/597

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Detection of Porcine Diarrhea Associated Virus and Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus in Hebei Province

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